Journal: Frontiers in Immunology

Article Title: Naringenin Modifies the Development of Lineage-Specific Effector CD4 + T Cells

doi: 10.3389/fimmu.2018.02267

Figure Lengend Snippet: Naringenin affects IL-6 receptor expression under iTreg-polarizing condition with IL-6 and inhibits IL-6 downstream signaling. (A–C) , Naïve CD4 + T cells from C57BL/6 mice were activated with anti-CD3/CD28 under iTreg-polarizing condition with IL-6 in the absence or presence of 80 μM naringenin. mIL-6R and mgp130 expression was determined by flow cytometry and sIL-6R levels in the cultured medium were quantified by ELISA. Values are mean ± SD of three independent experiments. Means without a common letter significantly differ at least at P < 0.05. * P < 0.05 compared with the corresponding control (without NAR) by student's t -test. D, After naïve CD4 + T cells were incubated with 80 μM naringenin for 2 h, IL-6 was added to stimulate cells for 15 min. Phosphorylation and acetylation of STAT3 expression were determined by Western blot. The gel pictures are representatives of three independent experiments, which had similar results. The values below band images are the ratios of Ac-STAT3/STAT3. NAR, naringenin.

Article Snippet: Cells were analyzed using flow cytometry as described above in “T cell division.” The concentrations of sIL-6Rα and sgp130 in the plasma samples mentioned above or supernatants from differentiated CD4 + T cells were quantified using the IL-6R and gp130 ELISA kit (Sino Biological Inc., Beijing) following the manufacturer's instruction.

Techniques: Expressing, Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay, Control, Incubation, Western Blot